INVESTIGATION OF GENETIC DIVERSITY OF WHEAT GENOTYPES (Triticum aestivum L.) USING MICROSATELLITE MARKERS

Abstract

The study was devoted to evaluate genetic diversity of 48 winter wheat genotypes (varieties
and advanced lines) with different origins using simple sequence repeat (SSR) markers. Out of 20
SSR primer pairs used for assessing the genetic diversity, 19 primer pairs were polymorphic. Microsatellite PIC values ranged from 0.26 to 0.93 with an average value of 0.740. The cluster analysis based on Nei genetic distance indices divided genotypes into four main groups. The Xwmc24,
Xwmc169, Xgpw322, Xgwm297 and Xbarc267 primers amplified high number of alleles, showing
relatively high level of polymorphism and evaluated as efficient primers for genetic diversity analysis of wheat populations.
Keywords: bread wheat, microsatellite, polyacrylamide gel, genetic diversity