INTERRELATIONS BETWEEN EPILEPTIC SEIZURES AND BRAIN NEURONS’ BACK REMODELING: CLINICAL AND EXPERIMENTAL STUDY

Abstract

The article concerns the problem of interrelationship between epileptic activity and brain neurons’ back remodeling process. The studies were carried out on chronic epileptic patients of pre-puberty period, of both sexes. Healthy persons of the same age served as a control group. The blood samples were taken from the patients in free-of-seizure timeframe and platelets and serum were saved. The levels of collapsin-response mediator protein 2 (CRMP2) and βIII tubulin were evaluated in the patients’ platelets and the levels of natural anti-CRMP2 autoantibodies and nerve growth factor (NGF) were evaluated in the patients’ serum with indirect ELISA test. Downregulation of CRMP2 and βIII tubulin in the platelets, sharp downregulation of natural anti-CRMP2 autoantibodies and upregulation of NGF in the serum was revealed. In the next series we studied the effect of intra-cerebral administration of CRMP2 to stress-tolerant Wistar male rats on their tolerance to the audiogenic stress effect (90-120 dB, 3 min). CRMP2 administration to the rats did not induce epileptic seizures under their exposure to audiogenic stress. The results give grounds to coming to a conclusion about engagement of brain neurons’ back remodeling in epileptic activity and that epileptic activity itself brings to induction of neurons’ back remodeling in the epileptogenesis-engaged neurons.